Brain nigrostriatal system changes in rotenone-induced parkinsonism (quantitative immune-morphological study)

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Abstract

For studying one of the commonest diseases of the nervous system, parkinsonism, long-term course of injections of pesticide rotenone to Wistar rats was used, and thereafter changes of neurons and glial cells in the nigrostriatal regions of the brain were investigated by immunohistochemical methods. It was found that rats treated by rotenone were characterized by reduced motor activity and displayed characteristics of experimental parkinsonism. These changes were accompanied by a decreased tyrosine hydroxylase staining in the processes of the s. nigra dopamine neurons and aggregation of α-synuclein in their bodies, as well as by significant loss of dopamine cells in the rostral part of the s.nigra. Rotenone produced bilateral local destruction of brain tissue with surrounding activated astrocytes in the dorsal parts of the striatum bilaterally. One may conclude that a parkinsonian model induced by rotenone is characterized by degenerative changes of dopamine neurons in the s. nigra, with α-synuclein aggregation and local and symmetrical injury of the striatum (with the involvement of dopaminergic fibers, neurons, neuroglia and cerebral vessels), which presumably reflects rotenone-induced mitochondrial dysfunction.

 
 

About the authors

Dmitriy N. Voronkov

Research Center of Neurology

Author for correspondence.
Email: voronkovdm@gmail.com
Russian Federation, Moscow

Yu. V. Dikalova

Research Center of Neurology

Email: platonova@neurology.ru
Russian Federation, Moscow

Rudolf M. Khudoerkov

Research Center of Neurology

Email: voronkovdm@gmail.com
Russian Federation, Moscow

Nina G. Yamshchikova

Research Center of Neurology

Email: voronkovdm@gmail.com
Russian Federation, Moscow

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Copyright (c) 2013 Voronkov D.N., Dikalova Y.V., Khudoerkov R.M., Yamshchikova N.G.

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