Annals of Clinical and Experimental NeurologyAnnals of Clinical and Experimental Neurology2075-54732409-2533Research Center of Neurology59010.25692/ACEN.2019.2.5UnknownInterhemispheric asymmetry of the cerebral amino acid pool in rat with subtotal cerebral ischaemiaRazvodovskyYury E.razvodovsky@tut.bySmirnovVitaliy Yu.razvodovsky@tut.byTroyanElina I.razvodovsky@tut.byMaksimovichNatalia E.razvodovsky@tut.byGrodno State Medical University25062019132414625062019Copyright © 2019, Razvodovsky Y.E., Smirnov V.Y., Troyan E.I., Maksimovich N.E.2019<p>The pathogenetic mechanisms of ischaemic stroke are complex and have not been fully studied, including the role of interhemispheric asymmetry in the brains biochemical organization.</p>
<p><strong>Study</strong> <strong>objective.</strong> To study the levels of free amino acids (AA) and their derivatives in the cerebral cortex of rats with subtotal cerebral ischaemia.</p>
<p><strong>Materials and methods.</strong> Subtotal cerebral ischaemia was modelled in 6 rats in the experimental group by ligation of both carotid arteries for 2 hours. Six rats with sham surgeries served as the control. The levels of AA and their derivatives was analysed in perchlorate tissue extract using reversed-phase chromatography.</p>
<p><strong>Results.</strong> Subtotal cerebral ischaemia was accompanied by changes in the AA pool, with differences found between the cortex of the left and right hemispheres. Glutamate, threonine, taurine, tyrosine, tryptophan and -aminoadipic acid levels decreased in the left frontal lobe cortex, and ornithine levels increased. Asparagine, serine and phenylalanine levels decreased in the right frontal lobe cortex.</p>
<p><strong>Conclusion.</strong> The nature of changes in the AA levels in the left and right halves of the cerebral cortex indicates interhemispheric asymmetry of amino acid imbalance, which develops in cerebral ischaemia.</p>cerebral hemispheressubtotal ischaemiaamino acidsasymmetryполушария мозгасубтотальная ишемияаминокислотыасимметрия[Feigin V.L., Norrving B., Mensah G.A. Global burden of stroke. Circ Res 2017; 120: 439–448. DOI: 10.1161/CIRCRESAHA.116.308413. PMID: 28154096.][Stakhovskaya L.V., Klotchichina О.А., Bogatyreva M.D., Kovalenko V.V. [Epidemiology of stroke in Russia according to results of territory-population register (2009–2010)]. Zhurnal nevrologii i psychiatrii 2013; 5: 1–10. (In Russ.)][Razvodovsky Yu.E. [Alcohol consumption and stroke mortality in Belarus]. Voprosy narcologii 2009; 6: 82–92. (In Russ.)][Razvodovsky Y.E. Alcohol-attributable fraction of stroke mortality in Russia. J Neurol Sci 2013; 333: e231. DOI: 10.1016/j.jns.2013.07.905][Gusev E.I., Skvortsova V.I. [Ischemia of the brain]. Мoscow, 2001. (In Russ.)][Maksimovich N.E. [Role of nitrogen oxide in the pathogenesis of ischemic reperfusion brain damage]. Grodno, 2004. (In Russ.)][Kulesh S.D. [Pathogenesis of ischemic stroke: biochemical mechanisms and role of neiroactive amino acids]. Meditsinskie novosti 1998; 1: 21–24. (In Russ.)][Zablocka B., Domanska-Janik K. Enhancement of 3[H]D-aspartate release during ischemia like conditions in rat hippocampal slices: source of excitatory amino acids. Acta Neurobiol Exp (Wars) 1996; 56: 63–70. PMID: 8787212.][Razvodovsky Y.E., Troyan E.I., Doroshenko Ye.M. et al. Levels of free amino acids and their derivates in the brain cortex of rats during unilateral ischemia. Int J Neurosci Behavior Sci 2017; 1 (1): 18–21.][Skvortsova V.I., Raevskiy K.S., Kovalenko A.V. [Concentration of neurotransmitters amino acids in the spinal cord liquid in patients with acute ischemic stroke]. Zhurnal nevrologii i psychiatrii 1999; 2: 34–38. (In Russ.)][Fernstrom J.D. Branched-chain amino acids and brain function. J Nutr 2005; (6 Suppl): 1539S–1546S. DOI: 10.1093/jn/135.6.1539S. PMID: 15930466.][Barcovskiy E.B., Bokun’ S.B., Borodinskiy A.N. et al. [Contemporary problems of biochemistry. Methods of investigation]. Minsk, 2013. (In Russ.)]