Annals of Clinical and Experimental NeurologyAnnals of Clinical and Experimental NeurologyАнналы клинической и экспериментальной неврологии2075-54732409-2533Eco-Vector13610.17816/psaic136ReviewsОбзорыUnknownNew capabilities of laboratory diagnosis of thromboses«Укутанные» тромбоциты – новые возможности лабораторной диагностики нарушений тромбообразованияhttps://orcid.org/0000-0001-9604-7775ShabalinaAlla A.ШабалинаАлла Анатольевна
Russian Federation

D. Sci. (Med.), leading researcher, Head, Laboratory of hemorheology, hemostasis and pharmacokinetics (with clinical laboratory diagnostics)

д.м.н., в.н.с., рук. лаб. гемореологии, гемостаза и фармакокинетики (с клинической лабораторной диагностикой)

ashabalina@yandex.ruKostyrevaM. V.КостыреваM. В.
Russian Federation
ashabalina@yandex.ruhttps://orcid.org/0000-0002-5883-8119TanashyanMarine M.ТанашянМаринэ Мовсесовна
Russian Federation

D. Sci. (Med.), Prof., Corresponding member of RAS, Deputy Director for science, Head, 1st Neurological department

д.м.н., профессор, член-корреспондент РАН, зам. директора по научной работе, рук. 1-го неврологического отделения

ashabalina@yandex.ruResearch Center of NeurologyФГБНУ «Научный центр неврологии»0909201593566001022017Copyright ©; 2015, Shabalina A.A., Kostyreva M.V., Tanashyan M.M.Copyright ©; 2015, Shabalina A.A., Kostyreva M.V., Tanashyan M.M.2015Shabalina A.A., Kostyreva M.V., Tanashyan M.M.Shabalina A.A., Kostyreva M.V., Tanashyan M.M.https://creativecommons.org/licenses/by/4.0https://annaly-nevrologii.com/pathID/article/view/136

The growing number of thromboses has been observed in various fields of medicine, which makes the issues of early diagnosis and investigation of thrombotic condition predictors more topical. Evaluation of hemostasis and investigation of risk factors of both thrombotic and hemorrhagic states is one of the most complex diagnostic problems. The objectives of laboratory diagnosis include not only studying of already known indicators of thrombovascular hemostasis and standard coagulation tests but also developing, automating, and improving of new methods for detection of biomarkers of hemostasis disturbances. According to the results of modern research, a high level of the subpopulation of procoagulant or coated platelets, known as COAT-platelets, in patient blood can be considered as this new marker for predicting thrombosis. COAT-platelets are a subpopulation of collagen and thrombin-activated platelets maintaining a high procoagulant activity of blood.

Обрисованы основные диагностические ориентиры использования методики определения субпопуляции «укутанных» тромбоцитов. Повышение их уровня более 45% ассоциируется с протромботическим потенциалом сосудисто-тромбоцитарного звена гемостаза и может быть предиктором развития тромботических состояний, повторных инсультов и ТИА, тогда как их сниженный (менее 20%) уровень – маркером развития кровотечений.

COAT-plateletshypercoagulationthrombosispredictor of thrombosis«укутанные» тромбоцитыгиперкоагуляциятромбозпредикторы тромбообразованияКозинец Г.И., Макаров В.А. Исследование системы крови в клинической практике. М.: Триада-Х, 1998; 454–455.Танашян М.М., Кузнецова П.И., Лагода О.В. и др. Миелопролиферативные заболевания и ишемический инсульт. Анналы клинич. и эксперим. неврологии. 2014; 8 (2): 41–45.Alberio L., Safa O., Clemetson K.J. et al. Surface expression and functional characterization of alpha-granule factor V in human platelets. Effects of ionophore A23187, thrombin, collagen and convulxin. Blood 2000; 95: 1694–1702.Behnke O, Forer A. Blood platelet heterogeneity. Evidence for two classes of platelets in man and rat. Br J Haematol 1993; 84: 686–693.Behnke O. Blood platelet heterogeneity: a functional hierarchy in the platelet population. Br J Haematol 1995; 91: 991–999.Brott T.G., Halperin J.L., Abbara S. et al. 2011 ASA/ACCF/AHA/AANN/AANS/ACR/ASNR/CNS/SAIP/SCAI/SIR/SNIS/SVM/ SVS Guideline on the Management of Patients With Extracranial Carotid and Vertebral Artery Disease A Report of the American College of Cardiology With the American Academy of Neurology and Society of Cardiovascular Computed Tomography. J Am Coll Cardiol. 2011. 57 (8): e16–94.Dale G.L., Friese P., Batar P. et al. Stimulated platelets use serotonin to enhance their retention of procoagulant proteins on the cell surface.Nature 2002, 415: 175–179.Dale G.L., Friese P., Batar P. et al. Stimulated platelets use serotonin to enhance their retention of procoagulant proteins on the cell surface. Nature 2002; 415: 175–179.DOI: 10.1038/415175a/Feng P., Tracy P.B. Not all platelets are equivalent procoagulants. Blood 1998; 92: 350a (Abstract).Heemskerk J.W., Mattheij N.J., Cosemans J.M. Platelet-based coagulation: different populations, different functions. J Thromb Haemost. 2012.Heemskerk J.W.M., Vuist W.M.J, Feijge M.A.H et al. Collagen but not fibrinogen surfaces induce bleb formation, exposure of phosphatidylserine, and procoagulant activity of adherent platelets: evidence for regulation by protein tyrosine kinase-dependent Ca2+ responses. Blood 1997; 90: 2615–2625.Holmes C.E., Bouchard B.A., Barbick R.R. et al. The existence of platelet subpopulations in patients with myeloproliferative disease: preliminary correlation with clinically abnormal hemostasis. Blood 2003;102: 787a (Abstract).Jackson S.P., Schoenwaelder S.M. Procoagulant platelets: are they necrotic? Blood 2010, 116: 2011–2018.Kempton C.L., Hoffman M., Roberts H.R., Monroe D.M. Platelet heterogeneity: variation on coagulation complex on platelet subpopulations. Arteriosclerosis, Thrombosis, and Vascular. Biology 2005, 25:861–866.60Том 9. № 3 2015Kirkpatrick А.С., Tafur A.J., Dale G.L., Prodan C.I. Coated-Platelets Improve Prediction of Stroke and Transient Ischemic Attack in Asymptomatic Internal Carotid Artery Stenosis Stroke. 2014 Oct; 45 (10):2995–3001. doi: 10.1161/STROKEAHA.114.006492. Epub 2014 Sep 2.Kulkarni S., Jackson S.P. Platelet factor XIII and calpain negatively regulate integrin alphaIIbbeta3 adhesive function and thrombus growth.J Biol Chem. 2004, 279 (29): 30697–30706.London F.S., Marcinkiewicz M., Walsh P.N. A subpopulation of platelets responds to thrombin- or SFLLRN-stimulation with binding site for factor IXa. Journ. of Biological Chem. 2004, 279 (19): 19854–19859.Mattheij N.J., Gilio K., Kruchten R.V. et al. Dual mechanism of integrin alphaIIbbeta3 closure in procoagulant platelets. J. Biol. Chem 2013.Munnix I.C., Kuijpers M.J., Auger J. et al. Segregation of platelet aggregatory and procoagulant microdomains in thrombus formation regulation by transient integrin activation. Arterioscler Thromb Vasc Biol.2007, 27: 1–7.Panteleev M.A., Ananyeva N.M., Greco N.J. et al. Two subpopulations of thrombin-activated platelets differ in their binding of the components of the intrinsic factor X-activating complex. Journal of Thrombosis and Haemostasis 2005, 3: 2545–2553.Patel D., Väänänen H., Jirousková M. et al. Dynamics of GPIIb/IIIamediated platelet–platelet interactions in platelet adhesion/thrombus formation on collagen in vitro as revealed by videomicroscopy. Blood 2003; 101: 929–936.Pasquet J.M., Dachary-Prigent J., Nurden A.T. Microvesicle release is associated with extensive protein tyrosine dephosphorylation in platelets stimulated by A23187 or a mixture of thrombin and collagen. Biochem J 1998; 333: 591–599.Pecci А., Carlo L. Balduini1 Platelets & thrombopoiesis: Desmopressin and super platelets March 20, 2014; Blood: 123 (12).Prodan C.I., Joseph P.M., Vincent A.S., Dale G.L. Coated-platelets in ischemic stroke: differences between lacunar and cortical stroke.J Thromb Haemost. 2008 Apr; 6 (4): 609–614. doi: 10.1111/j.1538-7836.2008.02890.x. Epub 2008 Jan 8.Prodan С.I., Stoner J.A., Cowan L.D., Dale G.L. Lower coatedplatelet levels are associated with early hemorrhagic transformation in patients with non-lacunar brain infarction Journ. of Thrombosis and Haemostasis Volume 8, Issue 6, p. 1185–1190, June 2010.Prodan C.I., Stoner J.A., Cowan L.D., Dale G.L. Higher coatedplatelet levels are associated with stroke recurrence following nonlacunar brain infarction.J Cereb Blood Flow Metab. 2013 Feb; 33 (2):287–292. doi: 10.1038/jcbfm.2012.168. Epub 2012 Nov 14.Prodan C.I., Stoner J.A., Dale G.L. Lower coated-platelet levels are associated with increased mortality after spontaneous intracerebral hemorrhage. Stroke 2015; 46: 1819–1825.Szasz R., Dale G.L. COAT platelets. Current opinion in hematology. 2003; 10: 351–355.Szasz R., Dale G.L. Thrombospondin and fibrinogen bind serotoninderivatized proteins on COAT-platelets. Blood 2002, 100: 2827–2831.Topalov N.N., Kotova Y.N., Vasil’ev S.A., Panteleev M.A. Identification of signal transduction pathways involved in the formation of platelet subpopulations upon activation. British Journal of Haematology 2012, 157 (1): 105–115.Valaydon Z.S., Lee P., Dale G.L. et al. Increased coated-platelet levels in chronic haemodialysis patients. Nephrology (Carlton) 2009, 14:148–154.Webber A.J., Firkin B.G. Two populations of platelets. Nature 1965;205: 1332.Weiss H.J., Hoyer L.W., Rickles F.R. et al. Quantitative assay of a plasma factor deficient in von Willebrand’s disease that is necessary for platelet aggregation. Relationship to factor VIII procoagulant activity and antigen content. J Clin Invest. 1973, 52 (11): 2708–2716.Yakimenko A.O., Verholomova F.Y., Kotova Y.N. et al. Identification of different proaggregatory abilities of activated platelet subpopulations. Biophys. J. 2012, 102, 2261–2269.