Annals of Clinical and Experimental NeurologyAnnals of Clinical and Experimental NeurologyАнналы клинической и экспериментальной неврологии2075-54732409-2533Eco-Vector145010.17816/ACEN.1450QROQMWReviewsОбзорыReview ArticleComparative analysis of methods used for assessing blood-brain barrier permeability with fluorescent probes in laboratory animalsСравнительный анализ методов оценки проницаемости гематоэнцефалического барьера с использованием флуоресцентных зондов у экспериментальных животныхhttps://orcid.org/0009-0007-4195-2533BerdnikovArseniy K.БердниковАрсений Константинович
Russian Federation

postgraduate student, Laboratory of neurobiology and tissue engineering, Brain Institute

аспирант лаб. нейробиологии и тканевой инженерии Института мозга

annaly-nevrologii@neurology.ruhttps://orcid.org/0000-0002-1284-6711AverchukAnton S.АверчукАнтон Сергеевич
Russian Federation

Cand. Sci. (Biol.), Assoc. Prof., senior researcher, Laboratory of neurobiology and tissue engineering, Brain Institute

канд. биол. наук, доцент, с. н. с. лаб. нейробиологии и тканевой инженерии Института мозга

annaly-nevrologii@neurology.ruhttps://orcid.org/0000-0001-5742-8356KomlevaYulia K.КомлеваЮлия Константиновна
Russian Federation

Dr. Sci. (Med.), leading researcher, Laboratory of neurobiology and tissue engineering, Brain Institute

д-р мед. наук, в. н. с. лаб. нейробиологии и тканевой инженерии Института мозга

annaly-nevrologii@neurology.ruhttps://orcid.org/0000-0001-9743-8700PotapenkoIlia V.ПотапенкоИлья Викторович
Russian Federation

PhD, researcher, Laboratory of neurobiology and tissue engineering, Brain Institute

PhD, н. с. лаб. нейробиологии и тканевой инженерии Института мозга

annaly-nevrologii@neurology.ruhttps://orcid.org/0000-0003-4012-6348SalminaAlla B.СалминаАлла Борисовна
Russian Federation

Dr. Sci. (Med.), Professor, Corr. Member of the RAS, principal researcher, Head, Laboratory of neurobiology and tissue engineering, Deputy director, Brain Institute

д-р мед. наук, профессор, член-корр. РАН, заместитель директора, г. н. с., зав. лаб. нейробиологии и тканевой инженерии Института мозга

annaly-nevrologii@neurology.ruRussian Center of Neurology and NeurosciencesРоссийский центр неврологии и нейронаук0704202620167751711202501122025Copyright ©; 2026, Berdnikov A.K., Averchuk A.S., Komleva Y.K., Potapenko I.V., Salmina A.B.Copyright ©; 2026, Бердников А.К., Аверчук А.С., Комлева Ю.К., Потапенко И.В., Салмина А.Б.2026Berdnikov A.K., Averchuk A.S., Komleva Y.K., Potapenko I.V., Salmina A.B.Бердников А.К., Аверчук А.С., Комлева Ю.К., Потапенко И.В., Салмина А.Б.https://creativecommons.org/licenses/by/4.0https://annaly-nevrologii.com/pathID/article/view/1450

Introduction. The blood-brain barrier (BBB) is a component of the neurovascular unit and a system regulating chemical homeostasis in brain tissue. Assessment of BBB permeability under normal and abnormal states of the central nervous system is of significant interest for experimental research in neuroscience. In recent years, there has been a substantial expansion of protocols that can be used to address such research tasks. Understanding the key differences, advantages, and limitations of each BBB investigation method is crucial for proper experimental design and data interpretation.

The aim of this review is to analyze modern methods for assessing BBB permeability in laboratory animals using fluorescent probes (tracers), provide their comparative characteristics, and evaluate selection criteria for solving experimental tasks.

Conclusion. Fluorescent probes enable real-time monitoring of BBB status in vivo during experimental neuroscience studies when assessing structural and functional integrity of the barrier in neuroinflammation, neurodegeneration, and brain tissue injury. Proper selection of fluorescent probes allows differentiated evaluation of para- and transcellular BBB permeability, vascular wall structure, and processes associated with brain tissue clearance.

Введение. Гематоэнцефалический барьер (ГЭБ) является компонентом нейроваскулярной единицы и системой, регулирующей химический гомеостаз в ткани головного мозга. Оценка проницаемости ГЭБ в норме и при патологических состояниях центральной нервной системы представляет значительный интерес при проведении экспериментальных исследований в области нейронаук. В последние годы существенно расширился спектр протоколов, которые могут быть использованы для решения таких исследовательских задач. Понимание ключевых различий, достоинств и ограничений каждого метода исследования ГЭБ важно для корректного проведения экспериментов и интерпретации полученных данных.

Цель обзора — анализ современных методов оценки проницаемости ГЭБ у экспериментальных животных с использованием флуоресцентных зондов (трейсеров), их сравнительная характеристика и оценка критериев выбора для решения экспериментальных задач.

Заключение. Использование флуоресцентных зондов предоставляет возможность регистрировать состояние ГЭБ in vivo в экспериментальных нейронауках при оценке структурно-функциональной целостности барьера при нейровоспалении, нейродегенерации, повреждении ткани головного мозга. Правильный выбор флуоресцентного зонда позволяет дифференцированно оценить состояние пара- и трансцеллюлярной проницаемости ГЭБ, структуру сосудистой стенки и процессы, связанные с клиренсом ткани головного мозга.

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