Annals of Clinical and Experimental NeurologyAnnals of Clinical and Experimental NeurologyАнналы клинической и экспериментальной неврологии2075-54732409-2533Eco-Vector29910.17816/psaic299Original articlesОригинальные статьиUnknownLaser-induced autofluorescence for assessment of methabolism and hemodynamic characteristics of the brainЛазер-индуцированная аутофлуоресценция для оценки метаболизма и гемодинамики головного мозгаSalminaA. B.СалминаA. Б.
Russian Federation
allasalmina@mail.ruSalminV. V.СалминВ. В.
Russian Federation
allasalmina@mail.ruFrolovaO. V.ФроловаO. В.
Russian Federation
allasalmina@mail.ruLaletinD. I.ЛалетинД. И.
Russian Federation
allasalmina@mail.ruFursovM. A.ФурсовM. A.
Russian Federation
allasalmina@mail.ruSkomorokhaD. P.СкоморохаД. П.
Russian Federation
allasalmina@mail.ruFursovA. A.ФурсовA. A.
Russian Federation
allasalmina@mail.ruKondrashovM. A.КондрашовM. A.
Russian Federation
allasalmina@mail.ruMedvedevaN. N.МедведеваН. Н.
Russian Federation
allasalmina@mail.ruMalinovskayaN. A.МалиновскаяН. A.
Russian Federation
allasalmina@mail.ruMantorovaN. S.МантороваН. С.
Russian Federation
allasalmina@mail.ruDepartment of Biochemistry, Medical, Pharmaceutical and Toxicological ChemistryНИИ молекулярной медицины и патобиохимииDepartment of Photonics and Laser Technologies, IEPRE, Siberian Federal UniversityИИФиРЭ ФГАОУ СФУDepartment of Histology and Embryology Krasnoyarsk State Medical University named after Prof. V.F.Voino-YasenetskyГОУ ВПО КрасГМУ им. проф. В.Ф. Войно-Ясенецкого1309201153323903022017Copyright ©; 2011, Salmina A.B., Salmin V.V., Frolova O.V., Laletin D.I., Fursov M.A., Skomorokha D.P., Fursov A.A., Kondrashov M.A., Medvedeva N.N., Malinovskaya N.A., Mantorova N.S.Copyright ©; 2011, Salmina A.B., Salmin V.V., Frolova O.V., Laletin D.I., Fursov M.A., Skomorokha D.P., Fursov A.A., Kondrashov M.A., Medvedeva N.N., Malinovskaya N.A., Mantorova N.S.2011Salmina A.B., Salmin V.V., Frolova O.V., Laletin D.I., Fursov M.A., Skomorokha D.P., Fursov A.A., Kondrashov M.A., Medvedeva N.N., Malinovskaya N.A., Mantorova N.S.Salmina A.B., Salmin V.V., Frolova O.V., Laletin D.I., Fursov M.A., Skomorokha D.P., Fursov A.A., Kondrashov M.A., Medvedeva N.N., Malinovskaya N.A., Mantorova N.S.https://creativecommons.org/licenses/by/4.0https://annaly-nevrologii.com/pathID/article/view/299

The paper contains results of the investigation of the laser-induced fluorescence detection method for the assessment of brain metabolism in situ through the dura mater. Models of anoxia and acute brain ischemia were used for the evaluation of reliability of the method utilizing registration of reduced tissue pyridine nucleotides fluorescence, as well as for the assessment of the viability index, based on the conversion of oxy- and deoxyhemoglobin. Some pathobiochemical mechanisms of alterations in the pool of pyridine nucleotides in anoxia and ischemia were analyzed.

В статье представлены результаты экспериментального исследования возможности применения метода лазерно-флуоресцентного анализа для оценки метаболизма ткани головного мозга in situ при сохранении целостности твердой мозговой оболочки. На модели аноксии и острой ишемии головного мозга продемонстрирована информативность метода регистрации флуоресценции восстановленных пиридиновых нуклеотидов и оценки индекса жизнеспособности, учитывающего конверсию окси- и дезоксиформ гемоглобина. Изучены некоторые патобиохимические механизмы изменения пула пиридиновых нуклеотидов при аноксии и ишемии.

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