Annals of Clinical and Experimental NeurologyAnnals of Clinical and Experimental NeurologyАнналы клинической и экспериментальной неврологии2075-54732409-2533Eco-Vector34010.17816/psaic340TechnologiesТехнологииUnknownNew technology in experimental neurobiology: neuronal networks coupled with multielectrode arrayНовые технологии в экспериментальной нейробиологии: нейронные сети на мультиэлектродной матрицеMukhinaI. V.МухинаИрина Владимировна
Russian Federation
haspekleon@mail.ruKhaspekovLeonid G.ХаспековЛеонид Георгиевич
Russian Federation
khaspekleon@mail.ruNizhny Novgorod State Medical AcademyНижегородская Государственная медицинская академияResearch Center of NeurologyФГБНУ «Научный центр неврологии»1306201042445103022017Copyright ©; 2010, Mukhina I.V., Khaspekov L.G.Copyright ©; 2010, Mukhina I.V., Khaspekov L.G.2010Mukhina I.V., Khaspekov L.G.Mukhina I.V., Khaspekov L.G.https://creativecommons.org/licenses/by/4.0https://annaly-nevrologii.com/pathID/article/view/340

Multielectrode arraуs (MEA) as the recording and stimulating system for self-organizing, functionally heterogeneous neuronal network formed by developing CNS cells in vitro is a new, unique technology for investigation of mechanisms of pathological neurodestructive processes and for searching the means of their pharmacological corrections. The principal advantages of the MEA are the precise noninvasive long-term network stimulation and measurement of the electric signals, pharmacological testing and drug screening, optical structural and functional imaging of metabolic ionic current into neurons and glial cells using confocal laser scanning microscopy. The brain cells and tissues coupled in vitro with MEA enables neuroprotective and/or neurotoxic drug testing in different models of the central nervous system pathological states. Data-intensive part, good reproducibility and quantitative assessment capability make it possible to relate the neuron networks cultured to the MEA with biosensors, allowing to perform effective pharmacological screening in vitro for the models of ischemia, trauma, epilepsy, Alzheimer’s disease, etc.

Мультиэлектродная система (Multielectrode Arraуs, или MEA система), регистрирующая in vitro нейронную активность в самоорганизующейся, функционально гетерогенной нейронной сети культивируемых клеток ЦНС, является новой уникальной технологией для выяснения закономерностей формирования межнейронных связей, а также исследования механизмов нейродеструктивных процессов и поиска способов их фармакологической коррекции. Основным преимуществом применения МЕА систем в электрофизиологических экспериментах является возможность длительной (в течение месяцев) неинвазивной регистрации сигналов и стимуляции культивируемых клеток мозга, в сочетании с прижизненной структурной и функциональной визуализацией ионных токов в нейронах и глиальных клетках in vitro с использованием лазерной сканирующей конфокальной микроскопии. Культивирование ткани и клеток мозга на МЕА системах позволяет в хроническом эксперименте тестировать соединения, оказывающие нейротоксический и нейропротекторный эффект. Большой объем и хорошая воспроизводимость получаемых результатов, а также возможность их количественной оценки позволяют отнести нейронные сети, культивируемые на МЕА системах, к биосенсорам, с помощью которых можно проводить эффективный фармакологический скрининг при моделировании in vitro различных форм нейродегенеративных заболеваний, таких, как ишемия, травма, эпилепсия, болезнь Альцгеймера и др.

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