Annals of Clinical and Experimental NeurologyAnnals of Clinical and Experimental NeurologyАнналы клинической и экспериментальной неврологии2075-54732409-2533Eco-Vector54510.25692/ACEN.2018.4.3Original articlesОригинальные статьиUnknownEvaluation of gut microbiota in Parkinson’s disease using gas chromatography with mass spectrometric detectionОценка микробиоты кишечника у пациентов с болезнью Паркинсона с помощью метода газовой хромато-масс-спектрометрииKrasakovIgor V.КрасаковИгорь Вячеславович
Russian Federation
ikrasakov@gmail.comhttps://orcid.org/0000-0001-8988-3011LitvinenkoIgor V.ЛитвиненкоИгорь Вячеславович
Russian Federation

D. Sci. (Med.), Professor, Head, Department of nervous diseases, Chief neurologist of the Russian Ministry of Defense

д.м.н., профессор, начальник каф. нервных болезней, главный невролог Минобороны России

ikrasakov@gmail.comRodionovGennadiy G.РодионовГеннадий Георгиевич
Russian Federation
ikrasakov@gmail.comShantyrIgor I.ШантырьИгорь Игнатьевич
Russian Federation
ikrasakov@gmail.comSvetkinaEkaterina V.СветкинаЕкатерина Владимировна
Russian Federation
ikrasakov@gmail.comThe Nikiforov All-Russian Centre of Emergency and Radiation MedicineФГБУ «Всероссийский центр экстренной и радиационной медицины им. А.М. Никифорова»S.M. Kirov Military Medical AcademyФГБВОУ ВО «Военно-медицинская академия им. С.М. Кирова»Nikiforov Russian Center of Emergency and Radiation MedicineФГБУ «Всероссийский центр экстренной и радиационной медицины им. А.М. Никифорова»07122018124232911122018Copyright ©; 2018, Krasakov I.V., Litvinenko I.V., Rodionov G.G., Shantyr I.I., Svetkina E.V.Copyright ©; 2018, Krasakov I.V., Litvinenko I.V., Rodionov G.G., Shantyr I.I., Svetkina E.V.2018Krasakov I.V., Litvinenko I.V., Rodionov G.G., Shantyr I.I., Svetkina E.V.Krasakov I.V., Litvinenko I.V., Rodionov G.G., Shantyr I.I., Svetkina E.V.https://creativecommons.org/licenses/by/4.0https://annaly-nevrologii.com/pathID/article/view/545

The paper presents preliminary results of a comparative study assessing the gut microbiota in patients with Parkinson's disease and the control group using the gas chromatography with mass spectrometric detection. Sixteen patients with stage 3 Parkinson's disease and 94 age-matched persons without Parkinson's disease were examined. It was revealed that the total number of microbial markers in parietal intestinal microbiota in patients with Parkinson's disease was increased by 43% compared with the control group. This increase is due to a 2-fold increase in the number of conditional-pathogenic flora, and at the same time there was a 2-fold decrease in the number of microbial markers of useful microflora. The obtained results may be regarded as preliminary and need to be assessed in a large cohort of patients with Parkinson’s disease. It is also necessary to assess the relationship between immune status and changes in microbiota, and to develop methods of correction of the revealed changes. Analysis of the efficiency of restoration of qualitative and quantitative composition of microbiota should be carried out using methods for the assessment of bioequivalence levodopa dose.

Представлены результаты сравнительного исследования состава микробиоты кишечника 16 пациентов с 3-й стадией болезни Паркинсона и 94 неврологически здоровых лиц сопоставимого возраста методом газовой хромато-масс-спектрометрии. В пристеночном слое кишечника у пациентов с болезнью Паркинсона общее количество микробных маркеров увеличено на 43% по сравнению с группой контроля. Увеличение происходит за счет повышения количества условно-патогенной флоры в 2 раза и уменьшения в 2 раза количества микробных маркеров полезной микрофлоры. Полученные результаты могут рассматриваться как промежуточные и нуждаются в валидации на репрезентативном числе пациентов. Необходимы также оценка взаимосвязи иммунного статуса с изменениями микробиоты и разработка методов коррекции выявленных изменений. Анализ эффективности восстановления качественного и количественного состава микробиоты должен проводиться с применением методов оценки биоэквивалентной дозы леводопы.

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