Annals of Clinical and Experimental NeurologyAnnals of Clinical and Experimental NeurologyАнналы клинической и экспериментальной неврологии2075-54732409-2533Eco-Vector7910.17816/psaic79ReviewsОбзорыUnknownModern tendencies in the development of the patch-clamp technique: new opportunities for neuropharmacology and neurobiologyСовременные тенденции в развитии метода локальной фиксации потенциала: новые возможности для нейрофармакологии и нейробиологииhttps://orcid.org/0000-0003-0078-4733ShuvaevAnton N.ШуваевАнтон Николаевич
Russian Federation

Head, Research Institute of Molecular Medicine and Pathobiochemistry

рук. НИИ молекулярной медицины и патобиохимии

shuvaevanton@hotmail.comSalminV. V.СалминВ. В.
Russian Federation
shuvaevanton@hotmail.comKuvachevaN. V.КувачеваН. В.
Russian Federation
shuvaevanton@hotmail.comPozhilenkovaE. A.ПожиленковаE. A.
Russian Federation
shuvaevanton@hotmail.comSalminaАlla B.СалминаАлла Борисовна
Russian Federation
shuvaevanton@hotmail.comKrasnoyarsk State Medical University named after Prof. V.F. Voino-YasenetskyФГБОУ ВО «Красноярский государственный медицинский университет имени профессора В.Ф. Войно-Ясенецкого»Voyno-Yasenetsky Krasnoyarsk State Medical UniversityФГБОУ ВО «Красноярский государственный медицинский университет имени профессора В.Ф. Войно-Ясенецкого»0712201594545831012017Copyright ©; 2015, Shuvaev A.N., Salmin V.V., Kuvacheva N.V., Pozhilenkova E.A., Salmina A.B.Copyright ©; 2015, Shuvaev A.N., Salmin V.V., Kuvacheva N.V., Pozhilenkova E.A., Salmina A.B.2015Shuvaev A.N., Salmin V.V., Kuvacheva N.V., Pozhilenkova E.A., Salmina A.B.Shuvaev A.N., Salmin V.V., Kuvacheva N.V., Pozhilenkova E.A., Salmina A.B.https://creativecommons.org/licenses/by/4.0https://annaly-nevrologii.com/pathID/article/view/79

The patch-clamp (PC) technique is the gold standard in studying processes that occur on the membranes of excitable cells, both in the physiological condition and in pathology. However, gaining this unique information is associated with certain difficulties. The technique is very labor intensive and requires highly qualified specialists and expensive equipment, which greatly limits its introduction into routine practice of a neurobiological laboratory. This article discusses the main advantages and disadvantages of PC as well as tendencies in improvement of the technique and its contribution to the development of neuropharmacology and neurobiology.

Метод локальной фиксации потенциала (МЛФП) – это золотой стандарт в исследовании процессов, проходящих на мембранах раздражимых клеток, как в физиологическом состоянии, так и при патологии. Однако получение такой уникальной информации связано с определенными трудностями. Этот метод очень трудоемок, требует высококвалифицированных специалистов и дорогостоящего оборудования, что значительно ограничивает его внедрение в широкую практику нейробиологической лаборатории. В статье рассматриваются основные достоинства и недостатки МЛФП, а также тенденции в усовершенствовании техники фиксации клеточной мембраны с помощью электродов и его вклад в развитие нейрофармакологии и нейробиологии.

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