Preconditioning as a method of neuroprotection in a model of brain infarct

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Preconditioning of ischemic and hypoxic type was investigated as a method of protecting brain against acute ischemic injury. The preconditioning methods were applied to experimental rats 24 h before the time when local brain infarct was done by middle cerebral artery occlusion (MCAO). It was found that the ischemic and hypoxic preconditioning resulted in three general morphological changes: 1) the size of infarct zone was reduced by 2.2–3.8 times compared with rats that had not been treated with the preconditioning before MCAO; 2) the preconditioning treatment retained the number of living neurons in penumbra at the level of control rats, while without the preconditioning neuronal count in the penumbra after MCAO was 29% lower; 3) the number of glial cells in penumbra was increased after MCAO by 38% compared with the control level, and continued to increase under the preconditioning treatment up to 60%, that suggests an important role of neuroglia in neuroprotection. Selective blockers of ATP2dependant K+2channels (52hydroxydecanoate and glibenclamide) completely abolished the neuroprotective effects of the preconditioning.


About the authors

Rudolf M. Khudoerkov

Research Center of Neurology

Author for correspondence.
Russian Federation, Moscow

N. S. Samojlenkova

M.V. Lomonosov Moscow State University

Russian Federation, Moscow

Svetlana A. Gavrilova

Lomonosov Moscow State University

Russian Federation, Moscow

Yu. A. Pirogov

M.V. Lomonosov Moscow State University

Russian Federation, Moscow

V. B. Koshelev

M.V. Lomonosov Moscow State University

Russian Federation, Moscow


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Copyright (c) 2009 Khudoerkov R.M., Samojlenkova N.S., Gavrilova S.A., Pirogov Y.A., Koshelev V.B.

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