Modern tendencies in the development of the patch-clamp technique: new opportunities for neuropharmacology and neurobiology

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Abstract

The patch-clamp (PC) technique is the gold standard in studying processes that occur on the membranes of excitable cells, both in the physiological condition and in pathology. However, gaining this unique information is associated with certain difficulties. The technique is very labor intensive and requires highly qualified specialists and expensive equipment, which greatly limits its introduction into routine practice of a neurobiological laboratory. This article discusses the main advantages and disadvantages of PC as well as tendencies in improvement of the technique and its contribution to the development of neuropharmacology and neurobiology.

About the authors

A. N. Shuvaev

V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk

Author for correspondence.
Email: platonova@neurology.ru
Russian Federation

V. V. Salmin

V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk

Email: platonova@neurology.ru
Russian Federation

N. V. Kuvacheva

V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk

Email: platonova@neurology.ru
Russian Federation

E. A. Pozhilenkova

V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk

Email: platonova@neurology.ru
Russian Federation

A. B. Salmina

V.F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk

Email: platonova@neurology.ru
Russian Federation

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Copyright (c) 2015 Shuvaev A.N., Salmin V.V., Kuvacheva N.V., Pozhilenkova E.A., Salmina A.B.

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