Modern tendencies in the development of the patch-clamp technique: new opportunities for neuropharmacology and neurobiology

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Abstract

The patch-clamp (PC) technique is the gold standard in studying processes that occur on the membranes of excitable cells, both in the physiological condition and in pathology. However, gaining this unique information is associated with certain difficulties. The technique is very labor intensive and requires highly qualified specialists and expensive equipment, which greatly limits its introduction into routine practice of a neurobiological laboratory. This article discusses the main advantages and disadvantages of PC as well as tendencies in improvement of the technique and its contribution to the development of neuropharmacology and neurobiology.

About the authors

Anton N. Shuvaev

Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky

Email: shuvaevanton@hotmail.com
ORCID iD: 0000-0003-0078-4733

Head, Research Institute of Molecular Medicine and Pathobiochemistry

Россия, 660022, Krasnoyarsk, Partizan Zheleznyak str., 1

V. V. Salmin

Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky

Email: shuvaevanton@hotmail.com
Россия, Krasnoyarsk

N. V. Kuvacheva

Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky

Email: shuvaevanton@hotmail.com
Россия, Krasnoyarsk

E. A. Pozhilenkova

Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky

Author for correspondence.
Email: shuvaevanton@hotmail.com
Россия, Krasnoyarsk

Аlla B. Salmina

Voyno-Yasenetsky Krasnoyarsk State Medical University

Email: shuvaevanton@hotmail.com
Россия, Krasnoyarsk

References

  1. Abd-Elsayed A.A., Ikeda R., Jia Z., et al. KCNQ channels in nociceptive cold-sensing trigeminal ganglion neurons as therapeutic targets for treating orofacial cold hyperalgesia. Mol. Pain. 2015; 11: 45.
  2. Adams M.E. Agatoxins: ion channel specific toxins from the american funnel web spider, Agelenopsis aperta. Toxicon. 2004; 43: 509–525.
  3. Blaesse P., Goedecke L., Bazelot M., et al. μ-Opioid Receptor-Mediated Inhibition of Intercalated Neurons and Effect on Synaptic Transmission to the Central Amygdala. J. Neurosci. 2015; 35: 7317–7325.
  4. Bordey A., Sontheimer H. Electrophysiological properties of human astrocytic tumor cells In situ: enigma of spiking glial cells. J. Neurophysiol. 1998; 79: 2782–2793.
  5. Bruno R.M., Sakmann B. Cortex is driven by weak but synchronously active thalamocortical synapses. Science. 2006; 312: 1622–1627.
  6. Cauli B., Tong X.K., Rancillac A. et al. Cortical GABA Interneurons in Neurovascular Coupling: Relays for Subcortical Vasoactive Pathways. J. Neurosci. 2004; 24: 8940–8949.
  7. Chen P., Zhang W., Zhou J. et al. Development of planar patch clamp technology and its application in the analysis of cellular electrophysiology. Progress in Natural Science. 2009; 19: 153–160.
  8. Cole K.S. Dynamic electrical characteristics of the squid axon membrane. Arch. Sci. Physiol. 1949; 3: 253–258.
  9. Epsztein J., Brecht M., Lee A.K. Intracellular determinants of hippocampal CA1 place and silent cell activity in a novel environment. Neuron. 2011; 70: 109–120.
  10. Epsztein J., Lee A.K., Brecht M. Impact of spikelets on hippocampal CA1 pyramidal cell activity during spatial exploration. Science. 2010; 327: 474–477.
  11. Fertig N., Blick R.H., Behrends J.C. Whole cell patch clamp recording performed on a planar glass chip. J. Biophys. 2002; 82: 3056–3062.
  12. Greifzu F., Pielecka-Fortuna J., Kalogeraki E., et al. Environmental enrichment extends ocular dominance plasticity into adulthood and protects from stroke-induced impairments of plasticity. Proc. Nat. Acad. Sci. USA. 2014; 111: 1150–1155
  13. Haider B., Häusser M., Carandini M. Inhibition dominates sensory responses in the awake cortex. Nature. 2013; 493: 97–100.
  14. Hamill O.P., Marty A., Neher E., et al. Improved patch-clamp techniques for high resolution current recording from cells and cell-free membrane patches. Pflügers Arch. 1981; 391: 85–100.
  15. Hodgkin A.L. and Huxley A.F. Currents carried by sodium and potassium through the membrane of the giant axon of Loligo. J. Physiol. 1952; 116: 449–472.
  16. Huxley A. Kenneth Sterward Cole 1900 – 1984. A biographical Memoir. Washington D.C. National Academies Press. 1996; 23-45.
  17. Kaneko Y., Matsumoto G., Hanyu Y. TTX resistivity of Na+ channel in newt retinal neuron. Biochem. Biophys. Res. Commun. 1997; 240: 651–656.
  18. Klausberger T., Somogyi P. Neuronal diversity and temporal dynamics: the unity of hippocampal circuit operations. Science. 2008; 321: 53–57.
  19. Kodandaramaiah S.B., Franzesi G.T., Chow B.Y. et al. Automated whole-cell patch-clamp electrophysiology of neurons in vivo. Nature Methods. 2012; 9: 585–587.
  20. Lee A.K., Manns I.D., Sakmann B., Brecht M. Whole-cell recordings in freely moving rats. Neuron. 2006; 51: 399–407.
  21. Lepple-Wienhues A., Ferlinz K., Seeger A., Schäfer A. Flip the tip: an automated, high quality, cost-effective patch clamp screen. Recept. Chan. 2003; 9: 13–17.
  22. Liguz-Lecznar M., Zakrzewska R., Kossut M. Inhibition of Tnf-α R1 signaling can rescue functional cortical plasticity impaired in early poststroke period. Neurobiol. Aging. 2015; pii: S0197-4580(15)00326-7.[Epub ahead of print] 23. Molleman A. Patch-clamping: an introductory guide to Patch-Clamp electrophysiology. 1st ed. John Wiley and Sons. 2003; 186.
  23. Sakmann B., Neher E. Single-channel recording. 2nd ed. Springer. 1995; 700.
  24. Sigworth F.J., Affolter H., Neher E. Design of the EPC-9, a computer-controlled patch-clamp amplifier. 2. Software. J. Neurosci Methods. 1995; 56: 203–215.
  25. Tao H., Santa A.D., Guia A., et al. Automated tight seal electrophysiology for assessing the potential hERG liability of pharmaceutical compounds. Assay Drug Dev. Tech. 2004; 2: 497–506.
  26. Tauc L., Frank K. The central neuron of Mollusca studied with the “voltage clamp” method. J. Physiol. 1962; 54: 415–416.
  27. The Nobel Prize in Physiology or Medicine 1991. nobelprize.org. Nobel Media AB. Retrieved November 8, 2014.
  28. Torregrosa R., Yang X.F., Dustrude E.T., et al. Chimeric derivatives of functionalized amino acids and α-aminoamides: Compounds with anticonvulsant activity in seizure models and inhibitory actions on central, peripheral, and cardiac isoforms of voltage-gated sodium channels. Bioorg. Med. Chem. 2015; 23: 3655–3666.
  29. Windhorst U., Johansson H. Modern techniques in neuroscience research. 1st ed. Springer. 1999; 1328.
  30. Wu L.J., Wu G., Akhavan Sharif M.R., et al. The voltage-gated proton channel Hv1 enhances brain damage from ischemic stroke. Nat. Neurosci. 2012; 15: 565–573.
  31. Zemancovics R., Kali S., Paulsen O. et al. Differences in subthreshold resonance of hippocampal pyramidal cells and interneurons: the role of h-current ana passive membrane characteristics. J. Physiol. 2010; 588: 2109–2132.

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Copyright (c) 2015 Shuvaev A.N., Salmin V.V., Kuvacheva N.V., Pozhilenkova E.A., Salmina A.B.

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