Morphological changes of the choroids plexus and ventricular walls of the cerebrum following experimental intraventricular haemorrhage

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Abstract

We assessed the morphological changes induced by intraventricular haemorrhage (IVH) and investigated the effects of intraventricular fibrinolytic treatment following IVH. The experiment was performed in 30 Wistar rats. In first (control) group (15 rats) animals received intraventricular injection of 40 microl autologous blood with 10 microl normal saline. In second group (15 rats) 40 microl autologous blood with 10 microl t-PA recombinant tissue plasminogen activator (t-PA) was injected in ventricular of the animals. Morphological examination of the brains were carried out 2 days and 21 days following IVH. Marked losses of the ependymal covering of the ventricular walls, choroids plexus were founded in bouth groups. The results indicate that intraventricular administered t-PA significantly enhanced the lysis of intraventricular blood clots, but r-PA (in second group) promoted delayed periventricular white matter edema formation (more 21 days).

 
 

About the authors

A. V. Shirshov

Research Center of Neurology

Email: platonova@neurology.ru
Россия, Moscow

Tatyana S. Gulevskaya

Research Center of Neurology

Email: platonova@neurology.ru
ORCID iD: 0000-0003-2970-8136

D. Sci. (Med.), Prof., chief researcher, Laboratory of neuromorphology

Россия, 125367, Moscow, Volokolamskoye shosse, 80

V. A. Morgunov

Research Center of Neurology

Email: platonova@neurology.ru
Россия, Moscow

V. G. Kucheryanu

Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences

Author for correspondence.
Email: platonova@neurology.ru
Россия, Moscow

References

  1. Колтовер А.Н., Верещагин Н.В., Людковская И.Г. и др. Патологическая анатомия нарушений мозгового кровообращения. М.: Медицина, 1975.
  2. Ширшов А.В., Добжанский Н.В., Пирадов М.А. Современные подходы к хирургическому лечению спонтанных кровоизлияний в мозг. В кн.: З.А. Суслина (ред.). Очерки ангионеврологии. М.: Атмосфера, 2005: 222–230
  3. Ширшов А.В. Супратенториальные гипертензивные внутримозговые кровоизлияния, осложненные острой обструктивной гидроцефалией и прорывом крови в желудочковую систему. Дисс… докт. мед. наук. М., 2006.
  4. Andaluz N., Zuccarello M., Wagner K.R. Experimental animal models of intracerebral hemorrhage. Neurosurg. Clin. N. Am. 2002; Jul., 13 (5): 385–393.
  5. Broderick J., Connolly S., Feldmann E. et al. Guidelines for the Management of Spontaneous Intracerebral Hemorrhage in Adults: 2007 Update: A Guideline From the American Heart Associiation/American Stroke Association Stroke Council, High Blood Pressure Research Council, and the Quality of Care and Outcomes in Research Interdisciplinary Working Group: The American Academy of Neurology affirms the value of this guideline as an educational tool forneurologists. Circulation 2007; 16: 391–413.
  6. Engelhard H.H., Andrews C.O., Slavin K.V. et al. Current management of intraventricular hemorrhage. Surg. Neurol. 2003; 60: 15–21.
  7. Graham D.I., Lantos P.I. (eds). Greenfield’s Neuropathology. 66th ed. London, 1997.
  8. Findlay J.M., Grace M.G.A., Weir B.K.A. Treatment of intraventricular hemorrhage with tissue plasminogen activator. Neurosurgery 1993; 32: 941–947.
  9. Febles Garcia P., Conde Garcia J., Lourido Garcia J. et al. Fibrinolitic treatment of cerebral intraventricular hemorrhage. Rev. Neurol. 2001; 33 (6): 544–547.
  10. Kano T., Harada T., Katayma J. Infiltration of tissue plasminogen activator through cerebral vessels: evaluation using a rat thrombocbolic atroke model. Acta Neurochir. Suppl. 2003; 86: 167–168.
  11. Kase C.S., Caplan L.R. Intracerebral hemorrhage. London: ButterworthhHeinemann, 1994.
  12. Mayfrank L., Kissler J., Raoofi R. et al. Ventricular dilatation in experimental intraventricular hemorrhage in pigs. Stroke 1997; 28: 141–148.
  13. Mayfrank L., Kim Y., Kissler J. et al. Morphological chages following experimental intraventricular haemorrhage and intraventriular fibrinolytic treatment with recombinant tissue plasminogen activator. ActaNeuropathol. (Berl.) 2000; 5 (100): 561–567.
  14. Naft F., Jenkins A., Mendelow A. et al. Early hemodynamic changes in experimental intracerebral hemorrhage. J. Neurosurg. 1986; 65: 697–703.
  15. Paxinos G., Wayson C. The Rat Brain in Stereotaxic Coordinates. 22d ed. NewwYork: Academic Press, 1986.
  16. Ripoll M.A., Raininko R. Experimental intracerebral and subarachnoid intraventricular haemorrhages. Acta Radiol. 2002; 43: 464–473.
  17. Rohde V., Rohde I., Thiex R. et al. Fibrinolysis therapy achieved with tissue plasminogen activator and aspiration of the liquefied clot after experimental intracerebral haemorrhage: rapid reduction in hematoma volume but intensification of delayed edema formation. J. Neurosurg. 2002; 97(4): 954–962.
  18. Thiex R., Weis J., Krings T. et al. Addition of intravenous NNmethyl DDaspartate receptor antagonists to local fibrinolytic therapy for the optimal treatment of experimental intracerebral hemorrhages. J. Neurosurg. 2007; 106: 314–320.
  19. Thiex R., Kuker W., Jungbluth P. et al. Minor inflammation after surgical evacuation compared with fibrinolytic therapy of experimental intracerebral hemorrhages. Neurol. Res. 2005; 27: 493–498.
  20. Thiex R., Kuker W., Muller H. et al. The longgterm effect of recombinant tissueeplasminogen – activator(rttPA) on edema formation in a largee animal model of intracerebral hemorrhage. Neurol Res. 2003; 25:254–262.
  21. Xi G., Keep R.F., Hoff J.T. Pathophysiology of brain edema formation. Neurosurg. Clin. N. Am. 2002; 13: 371–383.

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Copyright (c) 2008 Shirshov A.V., Gulevskaya T.S., Morgunov V.A., Kucheryanu V.G.

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