Interhemispheric asymmetry of the cerebral amino acid pool in rat with subtotal cerebral ischaemia

Cover Page

Cite item

Full Text


The pathogenetic mechanisms of ischaemic stroke are complex and have not been fully studied, including the role of interhemispheric asymmetry in the brain’s biochemical organization.

Study objective. To study the levels of free amino acids (AA) and their derivatives in the cerebral cortex of rats with subtotal cerebral ischaemia.

Materials and methods. Subtotal cerebral ischaemia was modelled in 6 rats in the experimental group by ligation of both carotid arteries for 2 hours. Six rats with sham surgeries served as the control. The levels of AA and their derivatives was analysed in perchlorate tissue extract using reversed-phase chromatography.

Results. Subtotal cerebral ischaemia was accompanied by changes in the AA pool, with differences found between the cortex of the left and right hemispheres. Glutamate, threonine, taurine, tyrosine, tryptophan and α-aminoadipic acid levels decreased in the left frontal lobe cortex, and ornithine levels increased. Asparagine, serine and phenylalanine levels decreased in the right frontal lobe cortex.

Conclusion. The nature of changes in the AA levels in the left and right halves of the cerebral cortex indicates interhemispheric asymmetry of amino acid imbalance, which develops in cerebral ischaemia.

About the authors

Yury E. Razvodovsky

Grodno State Medical University

Author for correspondence.
Belarus, Grodno

Vitaliy Yu. Smirnov

Grodno State Medical University

Belarus, Grodno

Elina I. Troyan

Grodno State Medical University

Belarus, Grodno

Natalia E. Maksimovich

Grodno State Medical University

Belarus, Grodno


  1. Feigin V.L., Norrving B., Mensah G.A. Global burden of stroke. Circ Res 2017; 120: 439–448. doi: 10.1161/CIRCRESAHA.116.308413. PMID: 28154096.
  2. Stakhovskaya L.V., Klotchichina О.А., Bogatyreva M.D., Kovalenko V.V. [Epidemiology of stroke in Russia according to results of territory-population register (2009–2010)]. Zhurnal nevrologii i psychiatrii 2013; 5: 1–10. (In Russ.)
  3. Razvodovsky Yu.E. [Alcohol consumption and stroke mortality in Belarus]. Voprosy narcologii 2009; 6: 82–92. (In Russ.)
  4. Razvodovsky Y.E. Alcohol-attributable fraction of stroke mortality in Russia. J Neurol Sci 2013; 333: e231. doi: 10.1016/j.jns.2013.07.905
  5. Gusev E.I., Skvortsova V.I. [Ischemia of the brain]. Мoscow, 2001. (In Russ.)
  6. Maksimovich N.E. [Role of nitrogen oxide in the pathogenesis of ischemic reperfusion brain damage]. Grodno, 2004. (In Russ.)
  7. Kulesh S.D. [Pathogenesis of ischemic stroke: biochemical mechanisms and role of neiroactive amino acids]. Meditsinskie novosti 1998; 1: 21–24. (In Russ.)
  8. Zablocka B., Domanska-Janik K. Enhancement of 3[H]D-aspartate release during ischemia like conditions in rat hippocampal slices: source of excitatory amino acids. Acta Neurobiol Exp (Wars) 1996; 56: 63–70. PMID: 8787212.
  9. Razvodovsky Y.E., Troyan E.I., Doroshenko Ye.M. et al. Levels of free amino acids and their derivates in the brain cortex of rats during unilateral ischemia. Int J Neurosci Behavior Sci 2017; 1 (1): 18–21.
  10. Skvortsova V.I., Raevskiy K.S., Kovalenko A.V. [Concentration of neurotransmitters amino acids in the spinal cord liquid in patients with acute ischemic stroke]. Zhurnal nevrologii i psychiatrii 1999; 2: 34–38. (In Russ.)
  11. Fernstrom J.D. Branched-chain amino acids and brain function. J Nutr 2005; (6 Suppl): 1539S–1546S. doi: 10.1093/jn/135.6.1539S. PMID: 15930466.
  12. Barcovskiy E.B., Bokun’ S.B., Borodinskiy A.N. et al. [Contemporary problems of biochemistry. Methods of investigation]. Minsk, 2013. (In Russ.)

Supplementary files

Supplementary Files

Copyright (c) 2019 Razvodovsky Y.E., Smirnov V.Y., Troyan E.I., Maksimovich N.E.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77-83204 от 12.05.2022.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies